lytC
168
major autolysin, N-acetylmuramoyl-L-alanine amidase, required for lateral cell wall growth and for flagellar function
locus
BSU_35620
Molecular weight
52.46 kDa
pI
10.11
function
major autolysin, cell separation, wall turnover
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
lytC, cwlB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2247 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,659,119 3,660,609
Phenotypes of a mutant
impaired in motility, the phenotype is suppressed by mutations in ''[gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]'' or ''[gene|A6E7212D159F076F5D26F9C02F340B40C3667623|smiA]'' [Pubmed|19542270]
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
Protein family
[wiki|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)
[wiki|Domains]
contains an amidase_3 domain (like [protein|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC], [protein|B09FB626274B81F00A4FB42D188E089095343182|cwlD], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ])
[wiki|MurNAc-LAA domain] (aa 322-490) (according to UniProt)
Structure
[PDB|5J72] (from Clostridium difficile, 32% identity) [pubmed|28132783]
[AF|Q02114]
[wiki|Localization]
secreted (according to Swiss-Prot)
for an analysis of motion of [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC], see [pubmed|38381561]
Expression and Regulation
Operons
genes
[gene|497C9654DC7514FF738D18E1F083299C43003A4C|lytA]-[gene|E1F9922DF7273040F84E5AC207A9F4CAFB85DC75|lytB]-[gene|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC]
description
[Pubmed|1357079]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: repression, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
[protein|920F91E748EE079FF864011D9052B073567C41E4|slrR]: repression, (in complex with [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]) [Pubmed|20351052], in [regulon|protein:920F91E748EE079FF864011D9052B073567C41E4|slrR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1357079], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|1357079], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
1A789 ( ''lytC''::''kan''), [Pubmed|1588906], available the [http://bgsc.org/getdetail.php?bgscid=1A789 Bacillus Genetic Stock Center]
BKE35620 ([gene|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGATTTCCTCCTTAAA, downstream forward: _UP4_TAATCGAAAGAGACAAATCT
BKK35620 ([gene|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGATTTCCTCCTTAAA, downstream forward: _UP4_TAATCGAAAGAGACAAATCT
References
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Time of last update: 2024-12-21 07:21:03
Author of last update: Jstuelk