opuE
168
proline transporter
locus
BSU_06660
Molecular weight
53.12 kDa
pI
9.81
function
compatible solute transport
product
proline transporter
essential
no
synonyms
opuE, yerK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0591 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
726,840 728,318
Phenotypes of a mutant
strong growth disadvantage in high-salinity minimal media lacking proline [Pubmed|22685134]
The protein
Protein family
[wiki|sodium:solute symporter (SSF) (TC 2.A.21) family] (according to UniProt)
Structure
[AF|O06493]
Paralogous protein(s)
[protein|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]
description
[Pubmed|22383849]
regulation
induced by glucose ([protein|search|CcpA]) [Pubmed|22900538]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: activation, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|9701821], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
GP4688 (trpC2 Δ[gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::neo), available in [wiki|Jörg Stülke]'s lab
available in [wiki|Erhard Bremer]'s lab [Pubmed|24142252]
BKE06660 ([gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGC
BKK06660 ([gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGC
labs
[wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-30 11:36:43
Author of last update: Robert.warneke