cpaA
168
K+/H+ antiporter
locus
BSU_11640
Molecular weight
67.29 kDa
pI
5.25
function
export of potassium ions
product
K+/H+ antiporter
essential
no
synonyms
cpaA, yjbQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0569 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,240,356 1,242,200
The protein
Protein family
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family (with [protein|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU], according to UniProt)
[wiki|Domains]
contains a [wiki|RCK_N domain] (aa 403-525) (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])
contains a [wiki|RCK_C domain] at the C-terminus (aa 533-614) (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt])
Structure
[PDB|4BWZ] (from Thermus thermophilus, corresponds to the transmembrane domain (aa 27 ... 402), 26% identity) [pubmed|23995679]
[PDB|4YS2] (S. aureus CpaA, the [wiki|RCK_C domain] in complex with c-di-AMP, 58% identity)
[AF|O31615]
Effectors of protein activity
binds c-di-AMP, which likely activates export activity [pubmed|31061098]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B163 (yjbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1162 NBRP B. subtilis, Japan]
GP2082 (''[gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]''::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE11640 ([gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG
BKK11640 ([gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG
Expression vectors
pGP2909 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP2438 ''yjbQ-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-28 22:04:11
Author of last update: Jstuelk