cpaA

cpaA
168

K+/H+ antiporter

locus
BSU_11640
Molecular weight
67.29 kDa
pI
5.25
Protein length
Gene length
function
export of potassium ions
product
K+/H+ antiporter
essential
no
synonyms
cpaA, yjbQ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0569 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,240,356  1,242,200
The protein
Protein family
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family (with [protein|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU], according to UniProt)
[wiki|Domains]
contains a [wiki|RCK_N domain] (aa 403-525) (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])
contains a [wiki|RCK_C domain] at the C-terminus (aa 533-614) (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt])
Structure
[PDB|4BWZ] (from Thermus thermophilus, corresponds to the transmembrane domain (aa 27 ... 402), 26% identity) [pubmed|23995679]
[PDB|4YS2] (S. aureus CpaA, the [wiki|RCK_C domain] in complex with c-di-AMP, 58% identity)
[AF|O31615]
Effectors of protein activity
binds c-di-AMP, which likely activates export activity [pubmed|31061098]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]
description
[Pubmed|22383849]
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[gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]

2024-09-13 02:40:34

Jstuelk

53

1c0e1b43e82f84b0b2840306a4b41efed56a5108

EC113DBD0DCC9C1470D5DF27A6FE92D8DA2FA354

Biological materials
Mutant
MGNA-B163 (yjbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1162 NBRP B. subtilis, Japan]
GP2082 (''[gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]''::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE11640 ([gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT,  downstream forward: _UP4_TAAATCTTGACCAAATAAGG
BKK11640 ([gene|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT,  downstream forward: _UP4_TAAATCTTGACCAAATAAGG
Expression vectors
pGP2909 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP2438 ''yjbQ-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
31361596,32095817,32472931,32603625
Original Publications
26171638,23995679,31061098

6985ED02AC7A04D4FD5E531C47DD4D1711D7F829

Page visits: 2568

Time of last update: 2024-10-12 03:29:42

Author of last update: Jstuelk