malR
168
two-component response regulator, regulation of malate uptake
locus
BSU_31530
Molecular weight
26.82 kDa
pI
5.98
function
regulation of malate uptake
product
two-component response regulator
essential
no
synonyms
malR, yufM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4565 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,238,751 3,239,458
Phenotypes of a mutant
unable to use malate as the single carbon source [Pubmed|12949159]
The protein
[wiki|Domains]
[wiki|Response regulatory domain] (aa 3-119) (according to UniProt)
Structure
[PDB|5LWK] (from Lactobacillus paracasei, N-terminal part, corresponds to aa 1 ... 122, 43% identity) [pubmed|28577341]
[AF|O05251]
Modification
phosphorylated by [protein|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK] on an Asp residue
Effectors of protein activity
phosphorylation likely affects DNA-binding activity
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|6FBBC2BDEAD72BEA0D9EA56668FDA5A3BE6F694E|malK]-[gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A635 (yufM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/635 NBRP B. subtilis, Japan]
GP1301 (cat), available in [wiki|Jörg Stülke]'s lab
BKE31530 ([gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATCATGATTTTCCTC, downstream forward: _UP4_TAAGAAAACGCACTGCTTGC
BKK31530 ([gene|689CF50691E77E5A40437EF1E6B5083AF7CFCE08|malR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATCATGATTTTCCTC, downstream forward: _UP4_TAAGAAAACGCACTGCTTGC
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
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Time of last update: 2024-12-30 11:04:03
Author of last update: Melvin.boenninger