pucE
168
xanthine dehydrogenase
locus
BSU_32470
Molecular weight
18.69 kDa
pI
6.13
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
pucE, yurB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2080 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,335,751 3,336,272
The protein
Catalyzed reaction/ biological activity
H2O + NAD+ + xanthine --> H+ + NADH + urate (according to UniProt)
H2O + hypoxanthine + NAD+ --> H+ + NADH + xanthine (according to UniProt)
[wiki|Domains]
2Fe-2S ferredoxin-type domain (aa 14-90) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|1JRO] (from ''Rhodobacter capsulatus'', 43% identity, 58% similarity) [Pubmed|11796116]
[AF|O32143]
Expression and Regulation
Operons
genes
[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
description
[Pubmed|12823818]
regulation
induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A939 (yurB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/939 NBRP B. subtilis, Japan]
BKE32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT, downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
BKK32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT, downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
References
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Time of last update: 2025-04-08 14:26:39
Author of last update: Melvin.boenninger