pksN
168
polyketide synthase of type I
locus
BSU_17210
Molecular weight
609.00 kDa
pI
5.471
function
polyketide synthesis
product
polyketide synthase of type I
essential
no
synonyms
pksN, pksP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4908 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,834,409 1,850,875
The protein
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
[wiki|Domains]
3 [wiki|Carrier domain]s (aa 983-1058, aa 2448-2525, aa 3952-4026) (according to UniProt)
Structure
[PDB|6MHL] (from B. amyloliquefaciens, corresponds to aa 1087 ... 1687, 2557 ...3181, 4077 ... 4686)
Expression and Regulation
Operons
genes
[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
description
[Pubmed|22383849]
regulation
expressed during the transition from growth to stationary phase ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB], [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|24187085]
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots [pubmed|34557426]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|24187085], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|24187085], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
additional information
this is a very large operon comprising about 75 kb
genes
[gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]-[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
description
[pubmed|22383849]
Biological materials
Mutant
BKE17210 ([gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTTTAAGATGTTCTT, downstream forward: _UP4_CTGTTATAGGAGGGGAAACG
BKK17210 ([gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTTTAAGATGTTCTT, downstream forward: _UP4_CTGTTATAGGAGGGGAAACG
References
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Time of last update: 2025-03-17 03:18:31
Author of last update: Jstuelk