citA

citA
168

minor [metabolite|citrate] synthase

locus
BSU_09440
Molecular weight
40.78 kDa
pI
5.78
Protein length
Gene length
function
unknown
product
minor [metabolite|citrate] synthase
essential
no
ec
2.3.3.1
synonyms
citA, citA1

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0372 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,021,057  1,022,157
The protein
Catalyzed reaction/ biological activity
[metabolite|acetyl-CoA] + H2O + [metabolite|oxaloacetate] --> [metabolite|citrate] + [metabolite|CoA] + H+ (according to UniProt)
Protein family
citrate synthase family (with [protein|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD] and [protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ], according to UniProt)
Structure
[PDB|2C6X]
[AF|P39119]
Paralogous protein(s)
[protein|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD], [protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|citZ]
Expression and Regulation
Operons
genes
[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]-[gene|EAEEA4DD9641919830A81185333A2610B964D37C|yhdF]
description
[Pubmed|22383849]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|11544224]
regulatory mechanism
[protein|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]: repression, [Pubmed|8045899], in [regulon|protein:E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8045898], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Open in new tab

[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]→[gene|EAEEA4DD9641919830A81185333A2610B964D37C|yhdF]

2024-10-02 03:18:36

ghost

65

1669cf6dea93935b9fe338732703c4f1a950412d

EC50BFD2C823FA692DE52CBF1B927D137552A855

Biological materials
Mutant
GP676 (erm), available in [wiki|Jörg Stülke]'s lab
GP1282 Δ''[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''cat'', available in [wiki|Jörg Stülke]'s lab
GP1753 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]'')::''aphA3'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
GP2360 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA])''::''erm'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
BKE09440 ([gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCCCCTATCC,  downstream forward: _UP4_TAACATATTTTGGCGTTTAT
BKK09440 ([gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCCCCTATCC,  downstream forward: _UP4_TAACATATTTTGGCGTTTAT
Expression vectors
pGP2516 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1287 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
8045899,8045898,22383849,39005358

6684F6083B001E9FDC5967799B715AE966B83E1D

Page visits: 3205

Time of last update: 2024-10-02 03:02:25

Author of last update: Jstuelk