dnaE
168
lagging strand DNA polymerase III (alpha subunit), part of the [wiki|replisome]
locus
BSU_29230
Molecular weight
125.14 kDa
pI
6.02
function
[category|SW.3.1.1|DNA replication](lagging strand)
product
lagging strand DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms
dnaE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0587 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,991,269 2,994,616
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for bacteriophage SPP1 replication [Pubmed|23268446]
Protein family
DNA polymerase type-C family (with [protein|48BC0FA9901F174472EB070C539EC75EC7C10E88|yorL] and [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC], according to UniProt)
Structure
[PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]
[AF|O34623]
[wiki|Localization]
forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
description
[pubmed|22383849]
regulation
induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [PubMed|14593098,16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
References
Reviews
Original Publications
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Time of last update: 2024-10-12 22:07:28
Author of last update: Jstuelk