des
168
phospholipid desaturase
locus
BSU_19180
Molecular weight
40.55 kDa
pI
9.88
function
adaptation of membrane fluidity at low temperatures
product
phospholipid desaturase
essential
no
synonyms
des, yocE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3239 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,089,396 2,090,454
Phenotypes of a mutant
cold-sensitive [Pubmed|19820084]
a strain lacking the [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] operon and the [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des] gene exhibits strongly reduced membrane fluidity and eventual growth arrest [pubmed|35037270]
The protein
Catalyzed reaction/ biological activity
introduces double bonds into long chain fatty acids [Pubmed|21665975]
Protein family
Fatty acid desaturase type 1 family (single member, according to UniProt)
[wiki|Cofactors]
[protein|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des] receives electrons from ferredoxin ([protein|4164F07C581320D67FDC1ED5014272B81702A371|fer]) or from either of the two flavodoxins, [protein|0932DDC285635C02067A3302CEDA302FE4269179|ykuN] or [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP], the three proteins can functionally replace each other [Pubmed|21665975]
Structure
[AF|O34653]
[wiki|Localization]
integral membrane protein [Pubmed|21665975]
Expression and Regulation
Operons
genes
[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
description
[Pubmed|10559169]
regulation
induced upon cold shock ([protein|search|DesR]) [Pubmed|11285232]
regulatory mechanism
[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]: activation, [Pubmed|11285232], [Pubmed|19820084], in [regulon|protein:F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10559169], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
in strains evolved for growth at low pressure, 'des' expression is significantly increased as compared to the wild type [PubMed|22416764]
Biological materials
Mutant
MGNA-A319 (yocE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/319 NBRP B. subtilis, Japan]
1A835 ( ''des''::''kan''), [Pubmed|11285232], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A835&Search=1A835 BGSC]
BKE19180 ([gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT, downstream forward: _UP4_TGATGATAAAGGAGGTCTTC
BKK19180 ([gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT, downstream forward: _UP4_TGATGATAAAGGAGGTCTTC
labs
[wiki|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 22:29:53
Author of last update: Jstuelk