glnR
168
transcriptional repressor ([wiki|MerR family]) of the [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] operon
locus
BSU_17450
Molecular weight
15.69 kDa
pI
9.73
function
regulation of glutamine synthesis
product
transcription repressor ([wiki|MerR family])
essential
no
synonyms
glnR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,877,959 1,878,366
The protein
Protein family
[wiki|MerR family]
[wiki|Domains]
[wiki|HTH merR-type domain] (aa 11-79) (according to UniProt)
[wiki|Cofactors]
feedback-inhibited [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] [Pubmed|18195355]
Structure
[PDB|4R4E] (the GlnR-DNA complex) [Pubmed|25691471]
[PDB|7TFC] (the [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]-[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] (C tail peptide) complex) [pubmed|35778410]
[AF|P37582]
Effectors of protein activity
activity is enhanced upon interaction of the C-terminal domain with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] [Pubmed|18331450]
Expression and Regulation
Operons
genes
[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]
description
[Pubmed|8636055]
regulation
expressed in the absence of glutamine ([protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]) [Pubmed|8636055]
the mRNA is processed between [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] and [gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, in complex with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2906311], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]-[gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB]-[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]
description
[Pubmed|22383849]
regulation
the mRNA is processed between [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] and [gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
genes
[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]
description
[Pubmed|22383849]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, in complex with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2906311], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
BP148 (del(''[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]'')::''cat''), available in [wiki|Fabian Commichau]'s lab
BKE17450 ([gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAATTTCCTCCTTTT, downstream forward: _UP4_TAATCCGTTAGCAACTGTTT
BKK17450 ([gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAATTTCCTCCTTTT, downstream forward: _UP4_TAATCCGTTAGCAACTGTTT
lacZ fusion
pGP189 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
References
Reviews
Original Publications
Page visits: 8375
Time of last update: 2024-12-08 09:21:56
Author of last update: Jstuelk