phoP
168
two-component response regulator ([wiki|OmpR family]), regulation of phosphate metabolism
locus
BSU_29110
Molecular weight
27.53 kDa
pI
5.07
function
regulation of phosphate metabolism ([gene|E0430C16C41440633647976A0F9183E24268E198|phoA], [gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB], [gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD], [gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resABCDE], [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]-[gene|911A4D957A6C0DFC636A152395119DBFC777DDA2|tagB], [gene|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagDEF], [gene|tuaA|tuaA-H])
product
two-component response regulator ([wiki|OmpR family])
essential
no
synonyms
phoP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,977,800 2,978,522
RNA
[wiki|Interactions]
[gene|8CF9A800BEEAE188D78B534F2444BD5F811457C2|phoS] - 5' UTR of [gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP] [pubmed|39472585]
The protein
Protein family
[wiki|OmpR family] of two-component response regulators
[wiki|Domains]
[wiki|Response regulatory domain] (aa 4-118) (according to UniProt)
Structure
[PDB|2OQR] (from Mycobacterium tuberculosis, 44% identity) [pubmed|17942407]
[PDB|1MVO] (receiver domain)
[AF|P13792]
Modification
phosphorylation by [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR] under conditions of phosphate limitation (stimulates DNA-binding activity)
Effectors of protein activity
phosphorylation stimulates DNA-binding activity
Paralogous protein(s)
[protein|6A37531896205A9B894C81AB0563C216C0B52CD7|ykoG], [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ], [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[gene|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR]
description
[Pubmed|14762014]
regulation
carbon catabolite repression ([protein|search|CcpA]) [Pubmed|16452408,12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|16452408,12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|20382764], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, due to [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]~P binding to the 3’end of the [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]-[protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|phoR] operon [Pubmed|25666134,15205429,14762014], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16452408], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15205429], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,15205429], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
additional information
translation of [gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP] is limited by sequestration of the ribosomal binding site, the RBS is released upon binding of the [gene|8CF9A800BEEAE188D78B534F2444BD5F811457C2|phoS] RNA [pubmed|39472585]
Biological materials
Mutant
1A966 ( ''phoP''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]
BKE29110 ([gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT, downstream forward: _UP4_TATAAACTGGAGGAGCCAAA
BKK29110 ([gene|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT, downstream forward: _UP4_TATAAACTGGAGGAGCCAAA
labs
[wiki|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]
References
Reviews
Original Publications
Targets of PhoR
Biochemical analyses
Page visits: 8015
Time of last update: 2024-11-22 23:34:14
Author of last update: Jstuelk