rpoE
168
[protein|search|RNA polymerase] delta subunit, affects the regulation of [wiki|RNA polymerase] by the concentration of the initiating nucleoside triphosphate (iNTP)
locus
BSU_37160
Molecular weight
20.25 kDa
pI
3.65
function
[wiki|transcription]
product
[wiki|RNA polymerase] delta subunit
essential
no
ec
2.7.7.6
synonyms
rpoE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3343 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,812,542 3,813,063
Phenotypes of a mutant
[protein|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE] is essential for cell survival when facing a competing strain in changing environment [Pubmed|23543716]
The protein
Catalyzed reaction/ biological activity
binds to DNA immediately upstream of the promoter element at A-rich sequences on the abrB and rrnB1 promoters and facilitates open complex formation [Pubmed|26546673]
represses expression of ''[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]'' [Pubmed|27679485]
Protein family
rpoE family (single member, according to UniProt)
[wiki|Domains]
HARE-HTH domain (aa 15-81) (according to UniProt)
Structure
[PDB|2M4K] (full-length protein) [Pubmed|23868186]
[PDB|2KRC] (N-terminal domain) [Pubmed|20310067]
[PDB|6ZFB] (RNA polymerase complex) [pubmed|33339827]
[AF|P12464]
[wiki|Localization]
closely associated with [wiki|RNA polymerase] involved in transcribing both mRNA and rRNA operons [Pubmed|20724389]
Expression and Regulation
Operons
genes
[gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]-[gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG]
description
[Pubmed|29794222]
regulation
the mRNA is processed between [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE] and [gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
genes
[gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]
description
[Pubmed|10336502]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17189250], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]-[gene|7D89BBB52403BF99416250688EFA90C2BE8EB591|acdA]-[gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]
description
[Pubmed|17189250]
regulation
repressed in the absence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]) [Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17189250], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
present at equimolar levels with [wiki|RNA polymerase] [PubMed|20724389]
Biological materials
Mutant
GP3216 ([gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
BKE37160 ([gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGTCGGACACTCCCTTT, downstream forward: _UP4_TAGTATCAGATTTCATCTTC
BKK37160 ([gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGTCGGACACTCCCTTT, downstream forward: _UP4_TAGTATCAGATTTCATCTTC
lacZ fusion
BP1145: Promotor-lacZ fusion, available at [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs
labs
[wiki|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
References
Reviews
Original Publications
Page visits: 5517
Time of last update: 2024-12-20 13:37:57
Author of last update: Jstuelk