ahrC
168
transcriptional regulator of the ArgR family, AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism
locus
BSU_24250
Molecular weight
16.69 kDa
pI
5.52
function
transcriptional regulator of arginine metabolic genes
product
transcriptional regulator (ArgR family)
essential
no
synonyms
ahrC, argR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1438 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,522,324 2,522,773
Phenotypes of a mutant
increased intracellular concentration of ornithine, citrulline, and arginine [pubmed|28679749]
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] inactivation allows growth of a [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA] [gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA] mutant at low potassium concentration [pubmed|28679749]
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] inactivation allows growth of a [gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA] mutant under conditions of osmotic stress (0.8 M NaCl) [pubmed|35783388]
The protein
Catalyzed reaction/ biological activity
transcriptional activator/ repressor of genes involved in arginine metabolism
Protein family
ArgR family (single member, according to UniProt)
[wiki|Cofactors]
L-arginine is the co-factor required for transcription repression/ activation [pubmed|18007040]
Structure
[PDB|2P5L] (complex with an 18bp DNA operator) [pubmed|18455186]
[PDB|2P5K] (N-terminus) [pubmed|18007039]
[PDB|2P5M] (C-Terminus) [pubmed|18007040]
[PDB|1F9N] [pubmed|11856827]
[AF|P17893]
Effectors of protein activity
L-arginine is the co-factor required for transcription repression/ activation [pubmed|18007040]
[wiki|Localization]
Cytoplasm
Additional information
Binding site consensus: CATGAATAAAAATKCAAK, where K=G or T, [pubmed|9383188] [pubmed|9383188]
Expression and Regulation
Operons
genes
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
description
[Pubmed|2106508]
regulation
by [protein|search|sRNA] [protein|search|sr1]
additional information
expression is fourfold increased upon depletion of ''[wiki|nusA]'' [http://www.nature.com/articles/nmicrobiol20157 Reference]
genes
[gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|tlyA]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
description
[pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
Other regulations
[protein|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]: translation inhibition
additional information
the [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA controls [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] expression via binding to the [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] mRNA, binding is enhanced by [gene|93F328524989597C7D2329B25E665496C9631E87|csrA] [pubmed|31043113]
Biological materials
Mutant
GP729 (''Δ''''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
GP3122 (''Δ''''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]''::''phleo''), available in [wiki|Jörg Stülke]'s lab
GP2185 (''Δ''''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]''::''ermC''), available in [wiki|Jörg Stülke]'s lab [pubmed|28679749]
BKE24250 ([gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAGCACCTCTATTTC, downstream forward: _UP4_TAACAGAAAATCTAACAAAG
BKK24250 ([gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAGCACCTCTATTTC, downstream forward: _UP4_TAACAGAAAATCTAACAAAG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Simon Phillips], Leeds University, UK [http://www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=SEVP Homepage]
[wiki|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
References
Reviews
Original Publications
Page visits: 8362
Time of last update: 2024-12-19 21:32:04
Author of last update: Robert.warneke