alaT
168
PLP-dependent L-alanine aminotransferase
locus
BSU_31400
Molecular weight
42.15 kDa
pI
5.11
function
biosynthesis of L-alanine
product
PLP-dependent L-alanine aminotransferase
essential
no
synonyms
alaT, yugH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,225,772 3,226,932
Phenotypes of a mutant
strongly impaired growth in the absence of alanine [pubmed|33155333]
a [gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT] [gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat] double mutant is auxotrophic for alanine [pubmed|33155333]
The protein
Catalyzed reaction/ biological activity
pyruvate + glutamate --> L-alanine + 2-oxoglutarate [pubmed|33155333]
Protein family
[wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
Structure
[PDB|1DJU] (from ''Pyrococcus horikoshii'', 47% identity) [Pubmed|10671523]
[AF|Q795M6]
Paralogous protein(s)
[protein|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB], [protein|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|patA]
Expression and Regulation
Operons
genes
[gene|1CA87275AFA1B4E4B5E8E10229009F785E70BBA8|alaR]-[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]
description
[Pubmed|22383849]
additional information
A [protein|search|ncRNA] is predicted between [gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI] and [gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT] [PubMed|20525796]
Biological materials
Mutant
GP3594 (Δ[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::''phleo''), available in [wiki|Jörg Stülke]'s lab
GP3575 (Δ[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::''kan''), available in [wiki|Jörg Stülke]'s lab
MGNA-A621 (yugH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/621 NBRP B. subtilis, Japan]
BKE31400 ([gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31400 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_ATAGTCTGATAAATACGAAG, downstream forward: _UP4_TAAAAAAAGATACGAACCTT
BKK31400 ([gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAGTCTGATAAATACGAAG, downstream forward: _UP4_TAAAAAAAGATACGAACCTT
References
Page visits: 3527
Time of last update: 2024-12-31 08:52:57
Author of last update: MBenda