rpsNB
168
alternative ribosomal protein S14*, can replace [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN] under conditions of zinc limitation
locus
BSU_08880
Molecular weight
10.20 kDa
pI
11.19
function
translation
product
alternative ribosomal protein S14*
essential
no
synonyms
rpsNB, rpsNB, yhzA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0199 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
965,909 966,178
The protein
Protein family
universal [wiki|ribosomal protein] uS14 family (with [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN], according to UniProt)
Structure
[PDB|5AFI] (the E. coli ribosome, 47% identity) [pubmed|25707802]
[AF|O31587]
Paralogous protein(s)
[protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN]
Expression and Regulation
Operons
genes
[gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]
description
[pubmed|22383849]
regulation
induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]) [PubMed|12904577,17163968]
part of the third wave of induction upon zinc depletion [Pubmed|27561249]
expression requires functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [PubMed|12904577,17163968], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
BKE08880 ([gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
BKK08880 ([gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT, downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
References
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Time of last update: 2024-10-03 01:15:26
Author of last update: Melvin.boenninger