sacC
168
levanase
locus
BSU_27030
Molecular weight
75.77 kDa
pI
6.79
function
degradation of levan to fructose
product
levanase
essential
no
ec
3.2.1.80
synonyms
sacC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1621 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,758,043 2,760,076
The protein
Catalyzed reaction/ biological activity
Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to UniProt)
Protein family
glycosyl hydrolase 32 family (with [protein|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB] and [protein|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA], according to UniProt)
[wiki|Domains]
glycosyl hydrolase 32 family domain, aa 30 - 513 [pubmed|23213210]
C-terminal carbohydrate-binding module, aa 515-677 [pubmed|23213210]
Structure
[PDB|1Y9M] (inulinase of Aspergillus awamori, glycosyl hydrolase 32 family domain, aa 30 - 513, 42% identity) [pubmed|15522299]
[PDB|4B1M] (the C-terminal carbohydrate-binding module, aa 515-677) [pubmed|23213210]
[AF|P05656]
Expression and Regulation
Operons
genes
[gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]
description
[Pubmed|2117666]
regulation
induced in the presence of fructose ([protein|search|LevR]) [Pubmed|1900939]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7592486], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR]: activation, [Pubmed|1900939], in [regulon|protein:1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|1924373], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
Biological materials
Mutant
BKE27030 ([gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTCCTTCGCTCCTC, downstream forward: _UP4_TAATTTTAAAAAAGGCACAG
BKK27030 ([gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTCCTTCGCTCCTC, downstream forward: _UP4_TAATTTTAAAAAAGGCACAG
References
Reviews
Research papers
Page visits: 3208
Time of last update: 2024-11-09 04:48:32
Author of last update: Melvin.boenninger