sacC

sacC
168

levanase

locus
BSU_27030
Molecular weight
75.77 kDa
pI
6.79
Protein length
Gene length
function
degradation of levan to fructose
product
levanase
essential
no
ec
3.2.1.80
synonyms
sacC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1621 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,758,043  2,760,076
The protein
Catalyzed reaction/ biological activity
Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to UniProt)
Protein family
glycosyl hydrolase 32 family (with [protein|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB] and [protein|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA], according to UniProt)
[wiki|Domains]
glycosyl hydrolase 32 family domain, aa 30 - 513 [pubmed|23213210]
C-terminal carbohydrate-binding module, aa 515-677 [pubmed|23213210]
Structure
[PDB|1Y9M] (inulinase of Aspergillus awamori, glycosyl hydrolase 32 family domain, aa 30 - 513, 42% identity) [pubmed|15522299]
[PDB|4B1M] (the C-terminal carbohydrate-binding module, aa 515-677) [pubmed|23213210]
[AF|P05656]
Expression and Regulation
Operons
genes
[gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]-[gene|CAE38D8F25A5EC6AC8045E40072E6FEC632045FA|levE]-[gene|8ABA3735067C7AA433E09F219EE7239196377A95|levF]-[gene|F23B951F60A6A669D5BD86836906E0F4AD7A0C1B|levG]-[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]
description
[Pubmed|2117666]
regulation
induced in the presence of fructose ([protein|search|LevR]) [Pubmed|1900939]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7592486], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR]: activation, [Pubmed|1900939], in [regulon|protein:1D2043CC0D8CF64142F0A5993A936C5A196726D4|levR regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|1924373], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
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[gene|75C568C8F6912AC064B8B435975D52510D25466A|levD]→[gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]

2024-10-09 22:08:19

ghost

86

d0f853318c4c7bee208c4a44dec93cde9661ecc4

0E14E80CBB0982955C34B99D35F549A378E3F088

Biological materials
Mutant
BKE27030 ([gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTCCTTCGCTCCTC,  downstream forward: _UP4_TAATTTTAAAAAAGGCACAG
BKK27030 ([gene|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|sacC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTCCTTCGCTCCTC,  downstream forward: _UP4_TAATTTTAAAAAAGGCACAG
References
Reviews
20735481,1784813
Research papers
15522299,23213210,1900939,2117666,7592486

61D5B957E018D019AE0CE1D5F6C6A33EF982ED49

Page visits: 3208

Time of last update: 2024-11-09 04:48:32

Author of last update: Melvin.boenninger