floT
168
membrane-associated scaffold protein, reduces membrane order, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with [protein|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]) resistance to cefuroxime
locus
BSU_31010
Molecular weight
55.82 kDa
pI
5.14
function
control of membrane fluidity
product
membrane-associated scaffold protein
essential
no
synonyms
floT, yuaG, yuaH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2268 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,180,465 3,181,994
Phenotypes of a mutant
delayed onset of sporulation, reduced sporulation frequency
defect in motility [Pubmed|22753055]
reduced [wiki|protein secretion] [Pubmed|23651456]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant does not induce [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a [wiki|sporulation] defect, due to the lack of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] [Pubmed|22882210]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]'' mutant displays a defective growth under oxygen-limiting conditions [Pubmed|25909364]
The protein
Catalyzed reaction/ biological activity
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]-dependent expression of ''[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]'' and the ''[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]
recruits [protein|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF] to focal assemblies [Pubmed|22753055]
controls protease activity of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] [Pubmed|24222488]
Protein family
Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) domain protein family (with [protein|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI] and [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]) [pubmed|34777311]
[wiki|Domains]
contains a SPFH domain (Stomatin, Prohibitin, Flotillin, and HflK/C-Domain) (aa 24 - 212) [pubmed|34777311]
Structure
[AF|O32076]
[wiki|Localization]
membrane associated [Pubmed|18763711]
co-localizes with [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA] and [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC] in discrete foci membrane [Pubmed|20713508]
forms discrete focal structures in the cytoplasma membrane [Pubmed|22753055]
6 foci [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] of are detected in strain 3610 [Pubmed|25909364]
Expression and Regulation
Operons
genes
[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]
description
[Pubmed|9987136]
regulation
expressed upon cell wall stress ([protein|search|SigW]) [Pubmed|9987136]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9987136], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
MGNA-A213 (yuaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/213 NBRP B. subtilis, Japan]
JS152 (markerless), available in [wiki|Daniel Lopez]'s lab
BKE31010 ([gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAAATTCCTCCTTTT, downstream forward: _UP4_GAGTAAGGAAAGGGCAGAAC
BKK31010 ([gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAAATTCCTCCTTTT, downstream forward: _UP4_GAGTAAGGAAAGGGCAGAAC
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]) for FloT [Pubmed|25909364], available in [wiki|Daniel Lopez]'s lab
GFP fusion
GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [wiki|Daniel Lopez]'s lab
JS280 (3610 ''amyE::floT-gfp''(spc)), available in [wiki|Daniel Lopez]'s lab
JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [wiki|Daniel Lopez]'s lab
JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [wiki|Daniel Lopez]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-20 10:27:26
Author of last update: Jstuelk