narH
168
nitrate reductase (beta subunit)
locus
BSU_37270
Molecular weight
55.30 kDa
pI
5.11
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (beta subunit)
essential
no
synonyms
narH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1140 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,824,806 3,826,269
The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
[wiki|Domains]
3 [wiki|4Fe-4S ferredoxin-type domain]s (aa 7-36, aa 172-203, aa 205-234) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [Pubmed|11289299]
Structure
[PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI] complex of E. coli, 59% identity) [pubmed|12910261]
[AF|P42176]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
description
[Pubmed|8846791,16428414]
regulation
expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
regulatory mechanism
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE37270 ([gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCATGACCATACCGATTT, downstream forward: _UP4_TAACTTTTGATAAATGAGGT
BKK37270 ([gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCATGACCATACCGATTT, downstream forward: _UP4_TAACTTTTGATAAATGAGGT
References
Reviews
Original Publications
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Time of last update: 2024-12-21 08:31:38
Author of last update: Jstuelk