nusB
168
transcription antitermination factor
locus
BSU_24320
Molecular weight
14.74 kDa
pI
4.76
function
stable RNA transcription
product
antitermination factor
essential
no
synonyms
nusB, yqhZ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0781 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,529,267 2,529,662
The protein
Protein family
nusB family (single member, according to UniProt)
Structure
[PDB|1EYV] (from Mycobacterium tuberculosis, 40% identity) [pubmed|10881194]
[PDB|3R2C] (the [protein|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]-[protein|751D0B909922D4D931FF3B2285CF142D966FCD48|rpsJ] complex from Aquifex aeolicus) [pubmed|21652641]
[AF|P54520]
Expression and Regulation
Operons
genes
[gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]-[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]
description
[Pubmed|2536750,11591660]
regulation
repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|2536750,11591660]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|2536750,11591660], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
Biological materials
Mutant
MGNA-C368 (yqhZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2366 NBRP B. subtilis, Japan]
BKE24320 ([gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT, downstream forward: _UP4_TAGGAGGAAAGAAAATGACT
BKK24320 ([gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT, downstream forward: _UP4_TAGGAGGAAAGAAAATGACT
References
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Time of last update: 2024-12-21 07:19:03
Author of last update: Jstuelk