sodA
168
superoxide dismutase, general stress protein, important for survival of ethanol and paraquat stresses and at low temperatures
locus
BSU_25020
Molecular weight
22.43 kDa
pI
5.2
function
detoxification of oxygen radicals
product
superoxide dismutase
essential
no
ec
1.15.1.1
synonyms
sodA, yqgD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0605 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,585,434 2,586,042
Phenotypes of a mutant
a [gene|913D6DA06785C3AD0C89D77D48831518C6663387|yqgC] [protein|6153A07B961017052E1072944B59F510D80CEC0F|sodA] double mutant is hypersensitive to [metabolite|Mn(2+)], due to reduced expression of [metabolite|Mn(2+)] exporters [protein|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP] and [protein|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS] and intracellular [metabolite|Mn(2+)] accumulation [pubmed|38819154]
The protein
Catalyzed reaction/ biological activity
2 H+ + 2 [metabolite|superoxide] --> H2O2 + O2 (according to UniProt)
Protein family
iron/manganese superoxide dismutase family (with [protein|E0645DD4A7A871457300139A7E5986EF11C07387|sodF], according to UniProt)
[wiki|Cofactors]
[metabolite|Mn(2+)]
Structure
[PDB|2RCV] [Pubmed|18084079]
[AF|P54375]
Modification
phosphorylation on Thr-34 AND Thr-70 [Pubmed|17218307]
Paralogous protein(s)
[protein|E0645DD4A7A871457300139A7E5986EF11C07387|sodF]
[wiki|Localization]
cytoplasm [Pubmed|22174384]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|913D6DA06785C3AD0C89D77D48831518C6663387|yqgC]-[gene|6153A07B961017052E1072944B59F510D80CEC0F|sodA]
description
[Pubmed|22383849]
genes
[gene|6153A07B961017052E1072944B59F510D80CEC0F|sodA]
description
[pubmed|22383849]
regulation
induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|15805528]
constitutive expression [Pubmed|22174384]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
GP3165 [gene|6153A07B961017052E1072944B59F510D80CEC0F|sodA]::spec trpC2 available in [wiki|Jrg Stlke]'s lab
MGNA-C451 (yqgD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2449 NBRP B. subtilis, Japan]
BKE25020 ([gene|6153A07B961017052E1072944B59F510D80CEC0F|sodA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAATTCCTCCTTAGT, downstream forward: _UP4_TAATGGCACAAACAAGGTCC
BKK25020 ([gene|6153A07B961017052E1072944B59F510D80CEC0F|sodA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAATTCCTCCTTAGT, downstream forward: _UP4_TAATGGCACAAACAAGGTCC
References
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Time of last update: 2025-01-28 14:40:31
Author of last update: Jstuelk