fabI
168
enoyl-acyl carrier protein reductase
locus
BSU_11720
Molecular weight
27.72 kDa
pI
5.61
function
fatty acid biosynthesis
product
enoyl-acyl carrier protein reductase
essential
no
ec
1.3.1.9
synonyms
fabI, yjbW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0623 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,247,788 1,248,564
The protein
Catalyzed reaction/ biological activity
2,3-saturated acyl-[ACP] + NAD+ --> (2E)-enoyl-[ACP] + H+ + NADH (according to UniProt)
Protein family
[wiki|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)
Structure
[PDB|3OIF] (complex with NAD and inhibitor triclosan) [Pubmed|21185310]
[AF|P54616]
Effectors of protein activity
inhibited by triclosan [Pubmed|11325930]
Paralogous protein(s)
[protein|5964B6E817260DA7937796DDFA753A665A04D650|fabL], one of the two proteins has to be present for viability [Pubmed|17114254]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]
description
[Pubmed|12737802]
regulation
expressed when the cells experience a lack of malonyl-CoA ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [Pubmed|12737802]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
sigma factors
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
Biological materials
Mutant
MGNA-B168 (yjbW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1167 NBRP B. subtilis, Japan]
BKE11720 ([gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
BKK11720 ([gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
References
Reviews
Original Publications
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Time of last update: 2024-10-03 23:59:12
Author of last update: Melvin.boenninger