rodZ
168
required for proper septum positioning during vegetative growth ([wiki|cell shape] determination), part of the [protein|search|Rod complex] for lateral [wiki|cell wall synthesis] and control of cell diameter
locus
BSU_16910
Molecular weight
23.48 kDa
pI
4.68
function
septum positioning during vegetative growth
product
morphogenic protein
essential
yes
synonyms
rodZ, ymfM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1426 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,761,707 1,762,573
Phenotypes of a mutant
essential [Pubmed|19164570,23879732]
depletion results in the formation of shorter and rounder cells [Pubmed|23879732]
depletion results in disturbed formation of asymmetric septa and perturbed [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF] activation [Pubmed|27415800]
hypersensitivity to sorbic acid stress [Pubmed|27818647]
small, round, DNA-containing cells [pubmed|29403445]
the mutant cells are thicker and longer than wild type cells [pubmed|34846166]
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The protein
Catalyzed reaction/ biological activity
the presence of [protein|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]-P results in increased [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] filament density and growth rate [pubmed|36797487]
[wiki|Domains]
N-terminal cytoplasmic domain (RodZn), a transmembrane domain, and C-terminal extra-cytoplasmic domain (RodZc) [Pubmed|25503291]
Structure
[PDB|3FYM] (from Staphylococcus aureus, corresponds to aa 1 ... 112, 31% identity)
[AF|O31771]
Modification
phosphorylation by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] in response to lipid II abundance [pubmed|36797487]
[wiki|Localization]
membrane protein (according to UniProt)
Additional information
overexpression results in growth inhibition in minimal medium (in the absence of Mg2+ ) [Pubmed|19164570]
RodZ is destabilized in a ''[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]'' mutant [Pubmed|23879732]
Expression and Regulation
Operons
genes
[gene|725094DC9353EBB5FD603AB389607D8C13DF5F51|ymfK/1]-[gene|9394EA0675DFFCB5F0ECD494C104A66CE13A8EF6|ymfK/2]-[gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B120 (ymfM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1119 NBRP B. subtilis, Japan]
BKE16910 ([gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG, downstream forward: _UP4_TAATTACCAGATGACTTTTC
BKK16910 ([gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG, downstream forward: _UP4_TAATTACCAGATGACTTTTC
labs
[wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
References
Reviews
Original Publications
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Time of last update: 2024-09-10 16:50:02
Author of last update: Jstuelk