pdaC

pdaC
168

peptidoglycan deacetylase C, confers lysozyme resistance to modified cell wall peptidoglycans

locus
BSU_12100
Molecular weight
53.67 kDa
pI
9.1
Protein length
Gene length
function
cell wall modification
product
polysaccharide deacetylase C
essential
no
synonyms
pdaC, yjeA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,281,128  1,282,531
Phenotypes of a mutant
sensitive to lysozyme treatment  [Pubmed|22277649]
overexpression prevents cell wall cleavage by [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] and [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [pubmed|34871030]
The protein
Catalyzed reaction/ biological activity
deacetylates N-acetylmuramic acid (MurNAc) but not GlcNAc from peptidoglycan [Pubmed|22277649]
Protein family
[wiki|polysaccharide deacetylase family] (according to UniProt)
[wiki|Domains]
aa 55 - 140:  DUF4163
aa 155 - 235: DUF3298
[wiki|NodB homology domain] (aa 278-452) (according to UniProt)
Structure
[PDB|6H8L] (the C-terminal family 4 carbohydrate esterase (CE4) catalytic domain) [pubmed|31690626]
[PDB|2C1G] (the NodB domain, from Streptococcus pneumoniae, 47% identity) [pubmed|16221761]
[PDB|3S5T] (the DUF3298 domain, from Bacteroides fragilis, 27% identity)
[AF|O34798]
Paralogous protein(s)
[protein|2E2D21BEA164C7EB93B6A3F3C990ABD475920A81|yheN], (33%)
[wiki|Localization]
cell membrane (single pass) [pubmed|31690626]
Expression and Regulation
Operons
genes
[gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]
description
[pubmed|22383849]
regulatory mechanism
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: repression, in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
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[gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]

2024-10-10 11:45:45

Bzhu

66

503d7be9d1b4cd984be3c844a55fd7897c2d1439

7B2BB6C3F1630A2080370377C3994774576BF132

additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-A355 (yjeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/355 NBRP B. subtilis, Japan]
BKE12100 ([gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC,  downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
BKK12100 ([gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC,  downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
References
17878218,17581128,22277649,23199363,26883633,16221761,29465029,31690626,34871030,38978453

603E226CE488A25C4E28A6A7363CCD65BE64BB21

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Time of last update: 2024-10-15 01:08:44

Author of last update: Jstuelk