pdaC
168
peptidoglycan deacetylase C, confers lysozyme resistance to modified cell wall peptidoglycans
locus
BSU_12100
Molecular weight
53.67 kDa
pI
9.1
function
cell wall modification
product
polysaccharide deacetylase C
essential
no
synonyms
pdaC, yjeA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,281,128 1,282,531
Phenotypes of a mutant
sensitive to lysozyme treatment [Pubmed|22277649]
overexpression prevents cell wall cleavage by [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] and [protein|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [pubmed|34871030]
The protein
Catalyzed reaction/ biological activity
deacetylates N-acetylmuramic acid (MurNAc) but not GlcNAc from peptidoglycan [Pubmed|22277649]
Protein family
[wiki|polysaccharide deacetylase family] (according to UniProt)
[wiki|Domains]
aa 55 - 140: DUF4163
aa 155 - 235: DUF3298
[wiki|NodB homology domain] (aa 278-452) (according to UniProt)
Structure
[PDB|6H8L] (the C-terminal family 4 carbohydrate esterase (CE4) catalytic domain) [pubmed|31690626]
[PDB|2C1G] (the NodB domain, from Streptococcus pneumoniae, 47% identity) [pubmed|16221761]
[PDB|3S5T] (the DUF3298 domain, from Bacteroides fragilis, 27% identity)
[AF|O34798]
Paralogous protein(s)
[protein|2E2D21BEA164C7EB93B6A3F3C990ABD475920A81|yheN], (33%)
[wiki|Localization]
cell membrane (single pass) [pubmed|31690626]
Expression and Regulation
Operons
genes
[gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]
description
[pubmed|22383849]
regulatory mechanism
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: repression, in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-A355 (yjeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/355 NBRP B. subtilis, Japan]
BKE12100 ([gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC, downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
BKK12100 ([gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACAAAAACTCCTTCTTTC, downstream forward: _UP4_TAAATTAGAAAAGGCTGTCC
References
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Time of last update: 2024-12-19 21:05:47
Author of last update: Jstuelk