bshB1
168
N-acetylglucosamine-malate deacetylase, involved in bacillithiol synthesis (major enzyme)
locus
BSU_22470
Molecular weight
24.66 kDa
pI
6.34
function
biosynthesis of bacillithiol
product
N-acetylglucosamine-malate deacetylase
essential
no
synonyms
bshB1, jojG, ypjG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2120 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,358,208 2,358,918
The protein
Catalyzed reaction/ biological activity
(S)-malyl N-acetyl-α-D-glucosaminide + H2O --> (S)-malyl α-D-glucosaminide + acetate (according to UniProt)
Protein family
PIGL family (with [protein|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2], according to UniProt)
[wiki|Cofactors]
Zn2+ [pubmed|31867856]
Structure
[PDB|6ULL] (complex with substrate analogue) [pubmed|31867856]
[PDB|6P2T] (complex with citrate) [pubmed|31867856]
[PDB|2IXD] (from B. cereus, 62% identity) [pubmed|17501983]
[AF|P42981]
Paralogous protein(s)
[protein|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]
''bshB1'' null mutant and ''[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]'' / ''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]'' double null mutant available in [wiki|John Helmann] lab
GP87 (''ypjG''::''pX2''(''cat'')), available in [wiki|Jörg Stülke]'s lab
BKE22470 ([gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG
BKK22470 ([gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG
References
Reviews
Original Publications
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Time of last update: 2024-10-15 03:45:56
Author of last update: Jstuelk