yaaJ

yaaJ
168

tRNA specific adenosine deaminase

locus
BSU_00180
Molecular weight
17.61 kDa
pI
5.64
Protein length
161 aaSequenceBlast
Gene length
486 bpSequenceBlast
function
tRNA modification
product
tRNA specific adenosine deaminase
essential
no
synonyms
yaaJ, tadA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0590 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
25,852  26,337
The protein
Catalyzed reaction/ biological activity
Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2) (according to UniProt)
adenosine34 in tRNA + H+ + H2O --> inosine34 in tRNA + NH4+ (according to UniProt)
Protein family
[wiki|Cytidine and deoxycytidylate deaminase family] (according to UniProt)
[wiki|Domains]
[wiki|CMP/dCMP-type deaminase domain] (aa 2-120) (according to UniProt)
Structure
[PDB|2B3J] (the protein of ''Staphylococcus aureus'', complex with RNA) [Pubmed|16415880]
[AF|P21335]
Expression and Regulation
Operons
genes
[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]-[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]
description
null
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, (both promoters), in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]→[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]

2024-09-24 10:03:09

ghost

108

b2d28cf402e740ffdb8d72c8cd99dd7c5f187533

7A6D368CD211CDB194D1980F4321F881B55AD15E

Biological materials
Mutant
MGNA-B891 (yaaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1890 NBRP B. subtilis, Japan]
BKE00180 ([gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT,  downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
BKK00180 ([gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT,  downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
References
19087206,12110595,16415880,28864459,37628567

5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB

Page visits: 4531

Time of last update: 2024-10-03 12:40:05

Author of last update: Jstuelk