yaaJ
168
tRNA specific adenosine deaminase
locus
BSU_00180
Molecular weight
17.61 kDa
pI
5.64
function
tRNA modification
product
tRNA specific adenosine deaminase
essential
no
synonyms
yaaJ, tadA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0590 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
25,852 26,337
The protein
Catalyzed reaction/ biological activity
Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2) (according to UniProt)
adenosine34 in tRNA + H+ + H2O --> inosine34 in tRNA + NH4+ (according to UniProt)
Protein family
[wiki|Cytidine and deoxycytidylate deaminase family] (according to UniProt)
[wiki|Domains]
[wiki|CMP/dCMP-type deaminase domain] (aa 2-120) (according to UniProt)
Structure
[PDB|7CPH] [Pubmed|34037063]
[PDB|2B3J] (the protein of ''Staphylococcus aureus'', complex with RNA, 61.7% identity) [Pubmed|16415880]
[AF|P21335]
Expression and Regulation
Operons
genes
[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]-[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]
description
null
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, (both promoters), in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B891 (yaaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1890 NBRP B. subtilis, Japan]
BKE00180 ([gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT, downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
BKK00180 ([gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT, downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
References
Page visits: 4636
Time of last update: 2024-12-21 23:27:50
Author of last update: Jstuelk