ansB
168
L-aspartase
locus
BSU_23570
Molecular weight
52.39 kDa
pI
5.69
function
[metabolite|aspartate] degradation
product
L-aspartase
essential
no
ec
4.3.1.1
synonyms
ansB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1027 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,454,347 2,455,774
The protein
Catalyzed reaction/ biological activity
L-[metabolite|aspartate] --> [metabolite|fumarate] + NH4+ (according to UniProt)
Protein family
class-II fumarase/aspartase family (with [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG], according to UniProt)
Structure
[PDB|3R6Q] (from ''Bacillus'' sp. YM55-1, 72% identity, 92% similarity) [Pubmed|21661762]
[AF|P26899]
Paralogous protein(s)
[protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]
Expression and Regulation
Operons
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
description
[Pubmed|1711029]
regulation
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Biological materials
Mutant
GP1153 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''ermC'') available in [wiki|Jörg Stülke]'s lab
GP4256 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''cat'') available in [wiki|Jörg Stülke]'s lab
BKE23570 ([gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
BKK23570 ([gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
lacZ fusion
pGP2274 (in [wiki|pAC5]) (GP2961), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-30 10:58:46
Author of last update: Jstuelk