opuAB
168
glycine betaine and arsenobetaine [wiki|ABC transporter] (permease)
locus
BSU_02990
Molecular weight
30.10 kDa
pI
9.84
function
compatible solute transport
product
glycine betaine and arsenobetaine [wiki|ABC transporter] (permease)
essential
no
synonyms
opuAB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4176 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
322,271 323,119
Phenotypes of a mutant
inactivation of [gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB] facilitates the adaptation a strain lacking c-di-AMP to growth on complex medium under anaerobic conditions [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
uptake of glycine betaine and arsenobetaine [pubmed|29159878]
uptake of dimethylglycine [Pubmed|24561588]
Protein family
[wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
[wiki|CysTW subfamily] (according to UniProt)
[wiki|Domains]
[wiki|ABC transmembrane type-1 domain] (aa 90-269) (according to UniProt)
Structure
[AF|P46921]
[wiki|Localization]
cell membrane [Pubmed|10092453,18763711]
Expression and Regulation
Operons
genes
[gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]-[gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]-[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]
description
[Pubmed|22383849]
regulation
induced by osmotic stress [Pubmed|23175650]
regulatory mechanism
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23175650], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE02990 ([gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGTATTCTAGGCAGTCTAT, downstream forward: _UP4_AAGAAAAGCAGGGGGAATGC
BKK02990 ([gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGGTATTCTAGGCAGTCTAT, downstream forward: _UP4_AAGAAAAGCAGGGGGAATGC
Expression vectors
pGP2925 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 20:52:23
Author of last update: Jstuelk