acoB
168
acetoin dehydrogenase E1 component (TPP-dependent beta subunit)
locus
BSU_08070
Molecular weight
36.69 kDa
pI
4.4
function
acetoin utilization
product
acetoin dehydrogenase E1 component (TPP-dependent beta subunit)
essential
no
synonyms
acoB, yfjJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0022 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
880,007 881,035
Phenotypes of a mutant
defective in the the ability to support the growth of ''Synechococcus leopoliensis'' CCAP1405/1 on agar media [Pubmed|25875741]
The protein
Structure
[PDB|3DUF] (PDH from Geobacillus stearothermophilus, 43% identity) [pubmed|19081062]
[AF|O34591]
Paralogous protein(s)
[protein|A024921961A786294199EA12E04456C890ED8D8C|bkdAB], [protein|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]
[wiki|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]-[gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]-[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]-[gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]
description
[pubmed|11274109]
regulation
induced by acetoin ([protein|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|706864AAF36684AD5E46F30E9EB76315AB412700|acoR]: activation, (interaction with [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]-containing [wiki|RNA polymerase]) [ PubMed|11274109], in [regulon|protein:706864AAF36684AD5E46F30E9EB76315AB412700|acoR regulon]
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: indirect positive regulation, in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [PubMed|11274109], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|21815947]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
MGNA-C281 (acoB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2279 NBRP B. subtilis, Japan]
BKE08070 ([gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTATGACTCTCGCCA, downstream forward: _UP4_GCATTGAATTAAAGGGGGGA
BKK08070 ([gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTATGACTCTCGCCA, downstream forward: _UP4_GCATTGAATTAAAGGGGGGA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
References
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Time of last update: 2024-12-23 23:57:38
Author of last update: LKrueger