yngI
168
aceto-acetyl-CoA ligase, may be involved in surfactin production
locus
BSU_18250
Molecular weight
61.26 kDa
pI
5.98
function
mother cell metabolism, leucine utilization
product
aceto-acetyl-CoA ligase
essential
no
ec
6.2.1.16
synonyms
yngI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,954,525 1,956,174
The protein
Catalyzed reaction/ biological activity
ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA [Pubmed|19935659]
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
Structure
[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 32% identity) [pubmed|22560731]
[AF|O31826]
Paralogous protein(s)
[protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA], [protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB], [protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]
Expression and Regulation
Operons
genes
[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
description
[Pubmed|12662922]
regulation
expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-B087 (yngI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1086 NBRP B. subtilis, Japan]
available in [wiki|Mohamed Marahiel]'s lab [Pubmed|20797616]
BKE18250 ([gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA
BKK18250 ([gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA
References
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Time of last update: 2024-10-02 20:46:45
Author of last update: Jstuelk