rpoY
168
epsilon subunit of [wiki|RNA polymerase]
locus
BSU_14540
Molecular weight
8.12 kDa
pI
4.61
function
control of [wiki|RNA polymerase] activity
product
epsilon subunit of [wiki|RNA polymerase]
essential
no
synonyms
rpoY, ykzG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5503 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,524,791 1,525,000
The protein
Protein family
UPF0356 family (single member, according to UniProt)
Structure
[PDB|4NJC] [Pubmed|25092033]
[AF|O31718]
[wiki|Localization]
colocalizes with the nucleoid [Pubmed|25092033]
Expression and Regulation
Operons
genes
[gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]-[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]
description
[Pubmed|24187087]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24187087], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE14540 ([gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA, downstream forward: _UP4_AAAGTATTGGAGTTATGAGC
BKK14540 ([gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA, downstream forward: _UP4_AAAGTATTGGAGTTATGAGC
Expression vectors
pGP2824: expression in ''B. subtilis'', with C-terminal Strep-tag, in [wiki|pGP382], available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP420 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-19 23:26:55
Author of last update: Jstuelk