ctpA
168
carboxy-terminal processing protease, promotes DNA damage checkpoint recovery
locus
BSU_19590
Molecular weight
50.98 kDa
pI
8.86
function
unknown
product
carboxy-terminal processing protease
essential
no
synonyms
ctpA, yzbD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0793 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,131,902 2,133,302
Phenotypes of a mutant
deletion of [protein|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA] and [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP] leads to accumulation of the checkpoint protein [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA] [pubmed|29979679]
sensitivity to DNA damage [pubmed|29979679]
The protein
Catalyzed reaction/ biological activity
digestion of [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA] [pubmed|29979679]
The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala (according to UniProt).
Protein family
Peptidase s41a family (together with [protein|85247AD09B7A472607B32D57E6318BA6C83EC6BA|ctpB], according to Uniprot)
[wiki|Domains]
signal peptide (aa 1 - 36) [pubmed|29979679]
[wiki|PDZ domain] (aa 96- 174) [pubmed|29979679]
S41 peptidase domain [pubmed|29979679]
peptidoglycan binding domain (C-terminal) [pubmed|29979679]
Structure
[PDB|4C2D] ([protein|85247AD09B7A472607B32D57E6318BA6C83EC6BA|ctpB], 42% identity) [pubmed|24243021]
[AF|O34666]
Paralogous protein(s)
[protein|85247AD09B7A472607B32D57E6318BA6C83EC6BA|ctpB]
[wiki|Localization]
cell membrane, with extracellular protease domain [pubmed|30315724]
Expression and Regulation
Operons
genes
[gene|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A087 (ctpA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/87 NBRP B. subtilis, Japan]
1A866 ( ''ctpA''::''cat''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A866&Search=1A866 BGSC]
BKE19590 ([gene|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAAACACCACCTTTTT, downstream forward: _UP4_TAAAAAAAACCATACGCGGC
BKK19590 ([gene|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAAACACCACCTTTTT, downstream forward: _UP4_TAAAAAAAACCATACGCGGC
References
Reviews
Original Publications
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Time of last update: 2024-12-21 22:07:59
Author of last update: Jstuelk