yloN

yloN
168

tRNA adenosine methyltransferase

locus
BSU_15750
Molecular weight
41.41 kDa
pI
6.79
Protein length
Gene length
function
RNA modification
product
tRNA methyltransferase
essential
no
synonyms
yloN, rlmN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0820 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,649,286  1,650,377
The protein
Catalyzed reaction/ biological activity
methylates C-2 in A2503 in 23S rRNA and A37 in tRNA [pubmed|23752511]
adenosine2503 in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine2503 in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)
adenosine37 in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine37 in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Radical SAM superfamily] (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|3RF9] (from E. coli, 38% identity) [pubmed|21527678]
[AF|O34617]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[Pubmed|16964327]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]→[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-10-06 16:52:51

Jstuelk

70

d80326080a97255f868ab0fdec860faa54d5674c

244CB061EA23DF7106827650C1723DD251F3D15F

genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
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[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]->[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-10-07 04:24:03

Jstuelk

130

67080d1e476b4c1544f46abe5db9b45c3ccd93f3

2C39B75EDCD294B0CAC1DECB4007285A35FDAE1D

Biological materials
Mutant
MGNA-B132 (yloN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1131 NBRP B. subtilis, Japan]
1A819 ( ''yloN''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A819&Search=1A819 BGSC]
BKE15750 ([gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA,  downstream forward: _UP4_CAAGACGAGACGAGGTGATG
BKK15750 ([gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA,  downstream forward: _UP4_CAAGACGAGACGAGGTGATG
References
18025251,18307109,27496281,23752511,11918677,21527678

5DEBD84E5937DB8B7F29C729FAA0076DF86A5451

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Time of last update: 2024-10-07 15:11:43

Author of last update: Jstuelk