fakA
168
fatty acid kinase
locus
BSU_15840
Molecular weight
59.32 kDa
pI
4.28
function
phosphorylation of fatty acids
product
fatty acid kinase
essential
no
synonyms
fakA, yloV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1461 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,656,442 1,658,103
The protein
Catalyzed reaction/ biological activity
phosphorylation of fatty acids (bound to [protein|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|degV] or [protein|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]) [pubmed|30429218]
[wiki|Domains]
kinase domain (DhaL domain) (aa 1-202) [pubmed|39454961]
middle (dimerization) domain (aa 239-321) [pubmed|39454961]
C-terminal domain (aa 333-548) [pubmed|39454961]
[wiki|Cofactors]
Zn (2+), binds to middle domain [pubmed|39454961]
Structure
[PDB|8VIP] (from S. aureus, N-terminal domain) (67% identity) [pubmed|39454961]
[PDB|7RM7] (from S. aureus, N-terminal domain) (67% identity)
[PDB|6W6B] (from S. aureus, C-terminal domain) (48% identity)
[AF|O34751]
Modification
phosphorylated on Arg-255 [Pubmed|22517742]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
Expression and Regulation
Operons
genes
[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B137 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1136 NBRP B. subtilis, Japan]
GP1466 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::''cat''), available in [wiki|Jörg Stülke]'s lab
GP1467 ([gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB] - [gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
BKE15840 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATAGACAATCCTACTCCTC, downstream forward: _UP4_TAGAAGGGCAATTTGCCCTT
BKK15840 ([gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATAGACAATCCTACTCCTC, downstream forward: _UP4_TAGAAGGGCAATTTGCCCTT
Expression vectors
pGP3623 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
pGP3624 expression of Strep-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA] by [wiki|pGP380] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3631 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP3643 (C-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP3780 [gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]-3xFLAG spc (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-22 08:24:21
Author of last update: Jstuelk