fabF
168
beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity
locus
BSU_11340
Molecular weight
43.84 kDa
pI
4.77
function
fatty acid biosynthesis
product
beta-ketoacyl-acyl carrier protein synthase II
essential
yes
ec
2.3.1.179
synonyms
fabF, yjaY
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0304 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,209,183 1,210,424
Phenotypes of a mutant
essential [Pubmed|12682299]
CRISPRi knock-down: terminal phenotype of growth cessation, moderate bending [Pubmed|27238023]
FabF-I109F: resistance to cerulenin [Pubmed|11325930,24641521]
FabF-L112F, FabF-I109M: resistance to cerulenin (Zeigler DR, unpublished observations)
The protein
Catalyzed reaction/ biological activity
(11Z)-hexadecenoyl-[ACP] + H+ + malonyl-[ACP] --> 3-oxo-(13Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP](according to UniProt)
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]-dependent expression of ''[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]'' and the ''[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]
Protein family
[wiki|thiolase-like superfamily] (according to UniProt)
Structure
[PDB|4LS5] [Pubmed|24641521]
[AF|O34340]
Expression and Regulation
Operons
genes
[gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]-[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]
description
[Pubmed|12737802]
regulation
''[wiki|fabHA]'': expressed when the cells experience a lack of malonyl-CoA ([protein|search|FapR]) [Pubmed|12737802]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12737802], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|21542858], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
BEC11340 (lacA::Pxyl-dcas9 amyE::Pveg-sgRNA(fabF) trpC2) [http://bgsc.org/getdetail.php?bgscid=BEC11340 BGSC], [Pubmed|27238023]
1A573 (fabF2 trpC2) [http://bgsc.org/getdetail.php?bgscid=1A573 BGSC], cerulenin-resistant mutant
1A576 (fabF14 trpC2) [http://bgsc.org/getdetail.php?bgscid=1A576 BGSC], cerulenin-resistant mutant
1A577 (fabF20 trpC2) [http://bgsc.org/getdetail.php?bgscid=1A577 BGSC], cerulenin-resistant mutant
References
Reviews
Original Publications
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Time of last update: 2024-10-14 23:45:48
Author of last update: Jstuelk