pyrF
168
orotidine 5-phosphate decarboxylase
locus
BSU_15550
Molecular weight
25.84 kDa
pI
5.84
function
pyrimidine biosynthesis
product
orotidine-5-phosphate decarboxylase
essential
no
ec
4.1.1.23
synonyms
pyrF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0284 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,628,622 1,629,341
Phenotypes of a mutant
poor growth [pubmed|28189581]
non-transformable [pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
H+ + orotidine 5'-phosphate --> CO2 + UMP (according to UniProt)
Protein family
OMP decarboxylase family (single member, according to UniProt)
Structure
[PDB|1DBT]
[AF|P25971]
Expression and Regulation
Operons
genes
[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
description
[Pubmed|8206849]
regulation
induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]
regulatory mechanism
[protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]: termination, via [wiki|RNA switch], in [regulon|protein:6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1709162], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE15550 ([gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAGTGTTTCTTCAGCTGACG, downstream forward: _UP4_GCCTATAAGGCTGTCAGACT
BKK15550 ([gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAGTGTTTCTTCAGCTGACG, downstream forward: _UP4_GCCTATAAGGCTGTCAGACT
References
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Time of last update: 2025-01-28 16:10:14
Author of last update: Melvin.boenninger