cwlC
168
N-acetylmuramoyl-L-alanine amidase
locus
BSU_17410
Molecular weight
27.01 kDa
pI
5.94
function
mother cell lysis
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
cwlC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3087 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,872,812 1,873,579
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
Protein family
[wiki|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)
[wiki|Domains]
contains an amidase_3 domain (like [protein|B09FB626274B81F00A4FB42D188E089095343182|cwlD], [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|yqiI], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|yrvJ])
[wiki|MurNAc-LAA domain] (aa 4-172) (according to UniProt)
SPOR domain (aa 180-254) (according to UniProt)
Structure
[PDB|1X60] (peptidoglycan binding domain) [pubmed|16042392]
[AF|Q06320]
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC]
description
[Pubmed|8407798]
regulation
expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|8407798]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|8407798], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
Biological materials
Mutant
BKE17410 ([gene|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCATCACCTCATTA, downstream forward: _UP4_TAGCCGAGACGGGGACGAGC
BKK17410 ([gene|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|cwlC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCATCACCTCATTA, downstream forward: _UP4_TAGCCGAGACGGGGACGAGC
References
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Time of last update: 2024-12-30 12:38:52
Author of last update: Jstuelk