rluB

rluB
168

ribosomal large subunit pseudouridine synthase B

locus
BSU_23160
Molecular weight
25.88 kDa
pI
10.25
Protein length
Gene length
function
23S rRNA modification
product
pseudouridine synthase
essential
no
ec
5.4.99.22
synonyms
rluB, ypuL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1187 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,421,438  2,422,172
The protein
Catalyzed reaction/ biological activity
uridine2605 in 23S rRNA --> pseudouridine2605 in 23S rRNA (according to E. coli RluB)
Protein family
[wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RsuA family (with [protein|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG], according to UniProt)
[wiki|Domains]
[wiki|S4 RNA-binding domain] (aa 2-68) (according to UniProt)
Structure
[PDB|4LAB] (from ''E. coli'', 40% identity) [Pubmed|24214967]
[AF|P35159]
Expression and Regulation
Operons
genes
[gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
description
[Pubmed|22383849]
Open in new tab

[gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]→[gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]

2024-12-21 12:00:32

Jstuelk

93

810e88212899b5da40206112287802e7d6377a71

48F6784365F7D14D1F54674DD0C766FFA75949EC

Biological materials
Mutant
MGNA-A106 (ypuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/106 NBRP B. subtilis, Japan]
BKE23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA,  downstream forward: _UP4_TAAATTTCACATAACCTTCA
BKK23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA,  downstream forward: _UP4_TAAATTTCACATAACCTTCA
References
36815589,9888802,24214967

5CD1E266B0EC82546A2AF19A05490DD4D04F812C

Page visits: 2070

Time of last update: 2024-12-20 20:30:15

Author of last update: Jstuelk