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MntR and
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gbsR
168
transcription repressor of the [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] and [gene|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA]-[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB] operons
Molecular weight
20.92 kDa
function
regulation of osmoprotection
product
transcription repressor
Genomic Context
This gene is a member of the following
regulons
Gene
Coordinates
3,186,763 3,187,305
The protein
Catalyzed reaction/ biological activity
sensing of choline and arsenocholine [Pubmed|29159878,22408163]
control of the [gene|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA]-[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB] and [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] operons [Pubmed|22408163]
Protein family
GbsR family (with [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|opcR] and [protein|4D288C63F68A9A4B39535D849D455B49F4E65050|yvaV], according to UniProt)
Structure
Effectors of protein activity
binds the inducer choline with an apparent K(D) of approximately 165 µM[Pubmed|22408163]
binds the inducer arsenocholine with an apparent K(D) of approximately 2.1 mM [Pubmed|29159878]
Expression and Regulation
Operons
genes
[gene|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR]
description
[Pubmed|22383849]
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[gene|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR]
2024-11-08 19:00:25
Jstuelk
61
5d064aabed5ab519971ec24f30f6300cf326d7d0
EF688366C2D2D15351200DCED1B726B368F893A2
Biological materials
Mutant
MGNA-A219 (yuaC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/219 NBRP B. subtilis, Japan]
BKE31070 ([gene|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGACCCTCTTTTCCT, downstream forward: _UP4_TAAAGCAGAAAACGCCTGGG
BKK31070 ([gene|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|gbsR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGACCCTCTTTTCCT, downstream forward: _UP4_TAAAGCAGAAAACGCCTGGG
lacZ fusion
pGP3876 (cat, based on [wiki|pAC5]]), available in [wiki|Jörg Stülke]'s lab
pGP3877 (kan, based on [wiki|pAC7]]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
References
22408163,28256787,29159878,32849357