pbpC
168
transpeptidase, penicillin-binding protein 3
locus
BSU_04140
Molecular weight
74.23 kDa
pI
6.2
function
unknown
product
class B penicillin-binding protein 3
essential
no
synonyms
pbpC, ycsM, yzsA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
463,934 465,940
Phenotypes of a mutant
increased sensitivity to several beta-lactams [pubmed|28792086]
The protein
Catalyzed reaction/ biological activity
cross-linking of glycan chains [pubmed|28792086]
Protein family
[wiki|transpeptidase family] (according to UniProt)
Structure
[PDB|5E31] (from Enterococcus faecium, 40% identity)
[AF|P42971]
[wiki|Localization]
mid cell localization (divisome), this depends on [protein|search|FtsZ ]and [protein|EBEFF9E0A524DCDA19382E5401B923B805E4C559|PBP2A ][pubmed|28792086]
extracellular (signal peptide) [Pubmed|18957862]
during vegetative growth: distinct foci and bands at cell periphery [pubmed|14731276]
localizes as patches in single cells and concentrates as a ring at the septum and the division site during the cell growth cycle [pubmed|32155044]
Expression and Regulation
Operons
genes
[gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]-[gene|D0A2506FDCEA41D2A8910AC2837B46E0B311D63B|ycsN]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE04140 ([gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
BKK04140 ([gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACTTTCCCCTGCCTTC, downstream forward: _UP4_TAAAATGTCTTTTTAAAAGG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jeff Errington] lab
GFP fusion
3105 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]::pSG5045 (cat Pxyl-gfpa-[gene|5BBA2769A9C0EDB56625F008A55470C2E2885AA4|pbpC]1-768) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
References
Reviews
Original Publications
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Time of last update: 2024-12-19 22:00:12
Author of last update: Jstuelk