rnmV
168
RNase M5
locus
BSU_00410
Molecular weight
20.53 kDa
pI
6.68
function
5S ribosomal RNA maturation
product
endoribonuclease M5
essential
no
synonyms
rnmV, yabF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1658 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
50,087 50,647
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor (according to UniProt)
Protein family
Ribonuclease M5 family (single member, according to UniProt)
[wiki|Domains]
N-terminal [wiki|TOPRIM domain] (aa 4-94) [Pubmed|33541205,9722641,16077031]
C-terminal NA-binding domain [pubmed|33541205]
[wiki|Cofactors]
two Mg2+ ions [pubmed|33541205]
Structure
[PDB|6TPQ] (from Geobacillus stearothermophilus, 61% identity) [pubmed|32991829]
[AF|P37547]
[wiki|Localization]
cytoplasm (homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]-[gene|FF329359CBE52C75A39237E8A6BB56F6AB321AFB|ksgA]
description
[Pubmed|11233981]
Biological materials
Mutant
MGNA-B906 (yabF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1905 NBRP B. subtilis, Japan]
BKE00410 ([gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTCCTCCAAGACAA, downstream forward: _UP4_GATACCGTTATGCGGGAGGA
BKK00410 ([gene|5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7|rnmV]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTCCTCCAAGACAA, downstream forward: _UP4_GATACCGTTATGCGGGAGGA
labs
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-10-03 10:56:52
Author of last update: Jstuelk