nfrA
168
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
locus
BSU_38110
Molecular weight
28.17 kDa
pI
5.73
function
unknown
product
FMN-containing NADPH-linked nitro/flavin reductase
essential
no
synonyms
nfrA, ywcG, ipa-43d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,911,477 3,912,226
The protein
Catalyzed reaction/ biological activity
possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD( ) degrading activity leading to free nicotinamide [Pubmed|20727352]
FMNH2 + NADP+ --> FMN + 2 H+ + NADPH (according to UniProt)
Protein family
flavin oxidoreductase frp family (with [protein|BA6C61C488659D5F1B5237BA817A8A5AC6D0E988|ycnD], according to UniProt)
[wiki|Cofactors]
FMN or FAD [Pubmed|21635694]
Structure
[PDB|3N2S] [Pubmed|20727352]
[AF|P39605]
Expression and Regulation
Operons
genes
[gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]-[gene|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH]
description
[Pubmed|10913069]
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|spx]) [pubmed|29271514]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|29271514,12642660,19575568], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10913069], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|9535080], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
MGNA-B232 (ywcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1231 NBRP B. subtilis, Japan]
BKE38110 ([gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT, downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT
BKK38110 ([gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT, downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT
References
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Time of last update: 2024-12-22 07:04:20
Author of last update: Melvin.boenninger