parA

parA
168

ATPase, centromere-like function involved in forespore chromosome partitioning / negative regulation of [category|SW.4.2|Sporulation] initiation, regulator of SMC complex dynamics

locus
BSU_40970
Molecular weight
27.39 kDa
pI
4.9
Protein length
253 aaSequenceBlast
Gene length
762 bpSequenceBlast
function
forespore chromosome partitioning / negative regulation of [category|SW.4.2|Sporulation] initiation
product
negative regulator of [category|SW.4.2|Sporulation] initiation
essential
no
synonyms
parA, soj, spo0JA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1192 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,206,397  4,207,158
Phenotypes of a mutant
defective in oriC segregation during [category|SW.4.2|Sporulation] [pubmed|27489185]
The protein
Catalyzed reaction/ biological activity
regulates [wiki|DNA replication] initiation by either inhibiting or activating the [wiki|DNA replication] initiator protein [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|21235642]; regulation of ''Spo0A'' expression [Pubmed|10852876]
has ATPase activity [pubmed|36301085]
Protein family
ParA family (with [protein|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|flhG] and [protein|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD], according to UniProt)
Structure
[PDB|5U1G] [pubmed|28373206]
[PDB|5U1J] [pubmed|28373206]
[AF|P37522]
Effectors of protein activity
[protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] inhibits [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] dimerization and concomitant DNA-binding activity by stimulating [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] ATPase activity [Pubmed|21235642]
[wiki|Interactions]
exists as ATP-bound dimer an ADP-bound monomer [pubmed|36301085]
[wiki|Localization]
polar/ septal at the cell membrane [Pubmed|20566861]
polar localization depends on the proton motive force [Pubmed|20566861]
Expression and Regulation
Operons
genes
[gene|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]-[gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]
description
[Pubmed|22383849]
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
Open in new tab

[gene|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]→[gene|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]

2024-12-14 11:35:18

ghost

103

86baea39c766e9113e6cde298217d7499c849707

0AEC202CC368218B597D878B7945E0D6E2DC147A

Biological materials
Mutant
BKE40970 ([gene|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGATGTCACCTACTTTCA,  downstream forward: _UP4_GATTTAGCAAAGGAAGTGGC
BKK40970 ([gene|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGATGTCACCTACTTTCA,  downstream forward: _UP4_GATTTAGCAAAGGAAGTGGC
labs
[wiki|Heath Murray], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]
References
Reviews
20157337,22934648,26286503,26706151,28075389,30863373,34220773,35638784,36825885,38723344
Original Publications
15659156,11532141,19450516,18854156,10619015,8866474,12492861,9701805,12950914,14651647,19450516,20566861,21235642,10852876,14563866,26253537,22286949,21911367,27059541,27489185,28373206,27489185,31318889,36206370,36301085,36815589

5B3DB5A796ACA48390278E60478DFF13D0074A8D

Page visits: 4898

Time of last update: 2024-12-19 15:04:39

Author of last update: Jstuelk