uppP
168
minor undecaprenyl pyrophosphate phosphatase
locus
BSU_31150
Molecular weight
30.19 kDa
pI
7.9
function
synthesis of undecaprenyl phosphate as precursor for lipid II
product
minor undecaprenyl pyrophosphate phosphatase
essential
no
ec
3.6.1.27
synonyms
uppP, yubB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1968 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,194,635 3,195,465
Phenotypes of a mutant
inactivation of ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' reduces sporulation efficiency to 0.4% that of wild type cells; distended intermembrane space [Pubmed|26735940]
a ''[gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]'' ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' double mutant is not viable (unless ''[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]'' is artificially expressed) [Pubmed|27528508]
The protein
Catalyzed reaction/ biological activity
di-trans,octa-cis-undecaprenyl diphosphate + H2O --> di-trans,octa-cis-undecaprenyl phosphate + H+ + phosphate (according to UniProt)
Protein family
uppP family (single member, according to UniProt)
Structure
[PDB|5OON] (from E. coli, 47% identity) [pubmed|29540682]
[AF|P94507]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Additional information
[protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP] seems to be a minor undecaprenyl pyrophosphate phosphatase, the major enzyme is [protein|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC] [Pubmed|22904278]
Expression and Regulation
Operons
genes
[gene|5B769207B05D93FEC36E97BB7074F29BB290C55A|yubA]-[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-A224 (yubB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/224 NBRP B. subtilis, Japan]
BKE31150 ([gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTTGATTCTCCCCA, downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC
BKK31150 ([gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTTGATTCTCCCCA, downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC
References
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Time of last update: 2024-12-29 20:16:57
Author of last update: Melvin.boenninger