uppP

uppP
168

minor undecaprenyl pyrophosphate phosphatase

locus
BSU_31150
Molecular weight
30.19 kDa
pI
7.9
Protein length
Gene length
function
synthesis of undecaprenyl phosphate as precursor for lipid II
product
minor undecaprenyl pyrophosphate phosphatase
essential
no
ec
3.6.1.27
synonyms
uppP, yubB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1968 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,194,635  3,195,465
Phenotypes of a mutant
inactivation of ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' reduces sporulation efficiency to 0.4% that of wild type cells; distended intermembrane space [Pubmed|26735940]
a ''[gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]'' ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' double mutant is not viable (unless ''[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]'' is artificially expressed) [Pubmed|27528508]
The protein
Catalyzed reaction/ biological activity
di-trans,octa-cis-undecaprenyl diphosphate + H2O --> di-trans,octa-cis-undecaprenyl phosphate + H+ + phosphate (according to UniProt)
Protein family
uppP family (single member, according to UniProt)
Structure
[PDB|5OON] (from E. coli, 47% identity) [pubmed|29540682]
[AF|P94507]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Additional information
[protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP] seems to be a minor undecaprenyl pyrophosphate phosphatase, the major enzyme is [protein|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC] [Pubmed|22904278]
Expression and Regulation
Operons
genes
[gene|5B769207B05D93FEC36E97BB7074F29BB290C55A|yubA]-[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]
description
[pubmed|22383849]
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[gene|5B769207B05D93FEC36E97BB7074F29BB290C55A|yubA]→[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]

2024-09-24 08:33:00

Jstuelk

66

fc06a9790a124297c70b0d099d77a06f77db736b

4F6B963370454EE3BF17CD5BB91E17D0A5A2245D

Biological materials
Mutant
MGNA-A224 (yubB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/224 NBRP B. subtilis, Japan]
BKE31150 ([gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTTTGATTCTCCCCA,  downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC
BKK31150 ([gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTTTGATTCTCCCCA,  downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC
References
18763711,22904278,26735940,27528508,29259598,29540682

5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99

Page visits: 3964

Time of last update: 2024-10-02 06:04:35

Author of last update: Melvin.boenninger