prfA

prfA
168

peptide chain release factor 1, recognize stop codon and hydrolyze the completed protein, RF1 recognizes UAA and UAG

locus
BSU_37010
Molecular weight
40.07 kDa
pI
4.82
Protein length
Gene length
function
translation
product
peptide chain release factor 1
essential
yes
synonyms
prfA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0216 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,797,085  3,798,155
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG [pubmed|11158343]
Protein family
prokaryotic/mitochondrial release factor family (with [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB], according to UniProt)
Structure
[PDB|1ZBT] (from ''Streptococcus mutans'', 58% identity)
[AF|P45872]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]-[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]-[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]
description
[Pubmed|22383849]
Open in new tab

[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]→[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]

2024-08-10 21:18:09

Jstuelk

79

f772f0238446923017cee65af9531adee8a533a8

11DDE5360DD627DCA1CFE914B4C14C3186501BC4

genes
[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]
description
[Pubmed|22383849]
Open in new tab

[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]

2024-10-04 12:27:48

Jstuelk

77

e504d55ad5c2f806d79431ce19d3414e3485fda2

934BB78F7FA03DDD2F648850A95155AA31D14293

References
Reviews
11158343
Original Publications
12237459,20512119,25217634,33900014

59E6468FD891F5271F38543515867B0B4625768D

Page visits: 2131

Time of last update: 2024-10-04 10:31:56

Author of last update: Jstuelk