prfA
168
peptide chain release factor 1, recognize stop codon and hydrolyze the completed protein, RF1 recognizes UAA and UAG
locus
BSU_37010
Molecular weight
40.07 kDa
pI
4.82
function
translation
product
peptide chain release factor 1
essential
yes
synonyms
prfA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0216 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,797,085 3,798,155
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG [pubmed|11158343]
Protein family
prokaryotic/mitochondrial release factor family (with [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB], according to UniProt)
Structure
[PDB|1ZBT] (from ''Streptococcus mutans'', 58% identity)
[AF|P45872]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]-[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]-[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]
description
[Pubmed|22383849]
genes
[gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]
description
[Pubmed|22383849]
References
Reviews
Original Publications
Page visits: 2233
Time of last update: 2024-12-18 21:36:38
Author of last update: Jstuelk