phoD
168
phosphodiesterase/alkaline phosphatase, degrades wall teichoic acid during phosphate starvation
locus
BSU_02620
Molecular weight
62.66 kDa
pI
8.39
function
aquisition of phosphate upon phosphoate starvation, degradation of wall teichoic acid during phosphate starvation
product
phosphodiesterase/alkaline phosphatase
essential
no
ec
3.1.3.1
synonyms
phoD, ycbS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3540 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
284,011 285,762
The protein
Catalyzed reaction/ biological activity
endohydrolytic activity on wall teichoic acid, acts in concert with [protein|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ] [Pubmed|27780866]
phosphate monoester + H2O --> alcohol + phosphate (according to UniProt)
Protein family
PhoD family (single member, according to UniProt)
[wiki|Cofactors]
one Fe3+ and two Ca2+ [Pubmed|25217636]
Structure
[PDB|2YEQ] [Pubmed|25217636]
[AF|P42251]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862], secreted by the [protein|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD] complex [Pubmed|19395490]
Expression and Regulation
Operons
genes
[gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]-[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[gene|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD]
description
[Pubmed|22383849]
regulation
expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|10094677]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|10094677,11007775], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10094677], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression of the operon depends on functional [protein|search|HemAT] and [protein|search|CsbC] [PubMed|23180473]
Biological materials
Mutant
BKE02620 ([gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC, downstream forward: _UP4_ATCACGAATTAAGGAGTGGA
BKK02620 ([gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC, downstream forward: _UP4_ATCACGAATTAAGGAGTGGA
labs
[wiki|Oscar Kuipers], University of Groningen, The Netherlands
[http://molgen.biol.rug.nl/molgen/index.php Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 13:38:26
Author of last update: Melvin.boenninger