ytpB
168
tetraprenyl-beta-curcumene synthase, catalyzes the first committed step in C35 terpenoid biosynthesis, salt stress protein
locus
BSU_30500
Molecular weight
42.68 kDa
pI
5.76
function
C35 terpenoid biosynthesis
product
tetraprenyl-beta-curcumene synthase
essential
no
synonyms
ytpB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,121,538 3,122,641
Phenotypes of a mutant
increased sensitivity to bacitracin, due to the accumulation of heptaprenyl pyrophosphate [Pubmed|24806199]
sensitivity is increased in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB] [gene|34DBBDD76A0B36867E5116FF17391DF70062F745|menA]'' double mutant [Pubmed|24806199]
The protein
Catalyzed reaction/ biological activity
all-trans-heptaprenyl diphosphate --> diphosphate + tetraprenyl-β-curcumene (according to UniProt)
Structure
[PDB|5YO8] (from Bacillus alcalophilus, 50% identity)
[AF|O34707]
Expression and Regulation
Operons
genes
[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
description
[Pubmed|9387221]
regulation
expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
genes
[gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]-[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
description
[Pubmed|18179421]
regulation
expressed under conditions of salt stress ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]) [pubmed|18179421]
induced by cell wall-inhibiting drugs, such as bacitracin and cefuroxime ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]) [pubmed|38323910]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
Biological materials
Mutant
MGNA-A164 (ytpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/164 NBRP B. subtilis, Japan]
BKE30500 ([gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACATGCACCCCCTACT, downstream forward: _UP4_AAAAATTCAAAGAAAAAAGC
BKK30500 ([gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACATGCACCCCCTACT, downstream forward: _UP4_AAAAATTCAAAGAAAAAAGC
References
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Time of last update: 2025-01-11 08:27:45
Author of last update: Jstuelk