pdhI
168
inhibitor of pyruvate dehydrogenase
locus
BSU_18060
Molecular weight
10.69 kDa
pI
3.67
function
control of pyruvate dehydrogenase activity
product
inhibitor of pyruvate dehydrogenase
essential
no
ec
null
synonyms
yneR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4841 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,931,545 1,931,832
The protein
Catalyzed reaction/ biological activity
binds to [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA] and [protein|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB] to inhibit pyruvate dehydrogenase activity [pubmed|36815589]
Protein family
HesB/IscA family (with [protein|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA], according to UniProt)
Structure
[AF|Q45063]
Expression and Regulation
Operons
genes
[gene|ADDEE0B251CBE61892482F15F91543242EDBF2A0|plsY]-[gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]
description
[Pubmed|22383849]
Biological materials
Mutant
GP3317 (Δ[gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE18060 ([gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAGAACACCCCTTTG, downstream forward: _UP4_TAAAAAAGAACGCACTTCAT
BKK18060 ([gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAGAACACCCCTTTG, downstream forward: _UP4_TAAAAAAGAACGCACTTCAT
Expression vectors
pGP3580 (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3588: expression of [gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-20 14:20:25
Author of last update: Jstuelk