sucD
168
succinyl-CoA synthetase (alpha subunit)
locus
BSU_16100
Molecular weight
31.23 kDa
pI
5.59
function
TCA cycle
product
succinyl-CoA synthetase (alpha subunit)
essential
no
ec
6.2.1.5
synonyms
sucD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0074 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,681,617 1,682,519
The protein
Catalyzed reaction/ biological activity
ATP + CoA + succinate --> ADP + phosphate + succinyl-CoA (according to UniProt)
Protein family
succinate/malate CoA ligase alpha subunit family (single member, according to UniProt)
Structure
[PDB|1JKJ] (E. coli)
[AF|P80865]
Modification
phosphorylation on (Ser-19 OR Thr-20) [Pubmed|17218307]
Effectors of protein activity
Inhibited by 2-oxoglutarate, ATP and NADH [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
GTP is not accepted by the enzyme [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
Kinetic information
Reversible Michaelis-Menten [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
Additional information
extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at [https://proteopedia.org/wiki/index.php/Succinyl-Coa_Synthetase Proteopedia]
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
description
[Pubmed|22383849]
regulation
repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12850135]
expression is heterogeneous [pubmed|29809139]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Biological materials
Mutant
GP720 (spc), available in [wiki|Jrg Stlke]'s lab
1A1008 ( [gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1008&Search=1A1008 BGSC]
GP791 ([gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD])::''tet'', available in [wiki|Jrg Stlke]'s lab
GP2344 ([gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD])::''kan'', Cre-recombinase is integrated in [gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA], available in [wiki|Jrg Stlke]'s lab
GP2345 ([gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]::''lox72''), Cre-recombinase is integrated in [gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA], available in [wiki|Jrg Stlke]'s lab
BKE16100 ([gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD])::erm trpC2 available at [http://bgsc.org/getdetail.php?bgscid=BKE16100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTGGTCCCCTGCCT, downstream forward: _UP4_TAATAAAAAAGGGACAGCCG
BKK16100 ([gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD])::kan trpC2 available at [http://bgsc.org/getdetail.php?bgscid=BKK16100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTGGTCCCCTGCCT, downstream forward: _UP4_TAATAAAAAAGGGACAGCCG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
FLAG-tag construct
GP1425 (spc, based on [wiki|pGP1331]), available in [wiki|Jrg Stlke]'s lab
Antibody
**
GFP fusion
GP1435 (spc, based on [wiki|pGP1870]), available in [wiki|Jrg Stlke]'s lab
References
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Time of last update: 2024-12-21 16:57:57
Author of last update: Jstuelk