ispC
168
1-deoxy-D-xylulose-5-phosphate reductoisomerase, second step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_16550
Molecular weight
43.12 kDa
pI
5.8
function
MEP pathway of isoprenoid biosynthesis
product
1-deoxy-D-xylulose-5-phosphate reductoisomerase
essential
yes
ec
1.1.1.267
synonyms
ispC, yluB, dxr
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0743 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,722,871 1,724,022
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
2-C-methyl-D-erythritol 4-phosphate + NADP+ --> 1-deoxy-D-xylulose 5-phosphate + H+ + NADPH (according to UniProt)
Protein family
DXR family (single member, according to UniProt)
Structure
[PDB|1R0K] (from ''Zymomonas mobilis'', 45% identity) [Pubmed|15063313]
[AF|O31753]
Expression and Regulation
Operons
genes
[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
description
[Pubmed|22383849]
genes
[gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]-[gene|7E7F6BBA03C443A5AFA05A6D9352FEF1212A5AF6|cdsA]-[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
description
[Pubmed|22383849]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Expression vectors
pGP4000: expression of [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-10-15 00:12:15
Author of last update: Robert.warneke