ureA
168
urease (gamma subunit)
locus
BSU_36660
Molecular weight
11.32 kDa
pI
4.98
function
utilization of urea as alternative nitrogen source
product
urease (gamma subunit)
essential
no
ec
3.5.1.5
synonyms
ureA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0831 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,768,791 3,769,108
The protein
Catalyzed reaction/ biological activity
2 H+ + H2O + urea --> CO2 + 2 NH4+ (according to UniProt)
Protein family
urease gamma subunit family (single member, according to UniProt)
Structure
[PDB|2FVH] (from ''Mycobacterium tuberculosis'', 57% identity, 62% similarity)
[AF|P75030]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|63CF8C8BBCE39BD585DD5F6C8BBD95E12B500AE6|ywzF]-[gene|1D9596609AEF10456041918BD592E49466D93B1F|ywzE]-[gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]
description
[Pubmed|22383849]
genes
[gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]
description
[Pubmed|9287005]
regulation
induced by nitrogen limitation ([protein|search|GlnR], [protein|search|TnrA]) [Pubmed|9287005]
regulatory mechanism
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, [Pubmed|9287005], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|9287005], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12374841], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|9287005,12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9287005], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|9287005], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
BKE36660 ([gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTAGTCCTCCTTCTT, downstream forward: _UP4_CCAATTTCTGCGGAGGTGAA
BKK36660 ([gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTAGTCCTCCTTCTT, downstream forward: _UP4_CCAATTTCTGCGGAGGTGAA
References
Reviews
Original Publications
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Time of last update: 2024-11-06 04:08:26
Author of last update: Melvin.boenninger